The fluxible
R package is made to transform any dataset of gas concentration over time measured with closed loop chamber systems into a gas flux dataset. Thanks to its flexibility, it works for all kinds of field setup (manual or automated chambers, tents, soil respiration chambers, …) and data collection strategies (separated files for each measurement vs continuous logging, variable vs constant chamber volume, variable vs constant measurement length, …). It is organized as a toolbox with one function per steps, which offers a lot of freedom and backwards compatibility for ongoing projects. If environmental data were recorded simultaneously (photosynthetically active radiation, soil temperature, …), they can also be processed (mean, sum or median), with the same focus window as the flux estimate.
The goal of fluxible
is to provide a workflow that removes individual evaluation of each flux, reduces risk of bias, and makes it reproducible. Users set specific data quality standards and selection parameters as function arguments that are applied to the entire dataset. fluxible
offers different methods to estimate fluxes: linear, quadratic, exponential (Zhao et al., 2018), and the original HM model (Hutchinson and Mosier, 1981; Pedersen et al., 2010). The kappamax method (Hüppi et al., 2018) is also included, at the quality control step. The package runs the calculations automatically, without prompting the user to take decisions mid-way, and provides quality flags and plots at the end of the process for a visual check.
This makes it easy to use with large flux datasets and to integrate into a reproducible and automated data processing pipeline such as the targets
R package (Landau, 2021). Using the fluxible
R package makes the workflow reproducible, increases compatibility across studies, and is more time efficient.
For a visual overview of the package, see the poster.
Installation
fluxible
can be installed from CRAN.
install.packages("fluxible")
You can install the development version of fluxible
from the GitHub repo with:
# install.packages("devtools")
devtools::install_github("plant-functional-trait-course/fluxible")
Short example
library(fluxible)
conc_df <- flux_match(
co2_df_short,
record_short,
datetime,
start,
measurement_length = 220
)
slopes_df <- flux_fitting(
conc_df,
conc,
datetime,
fit_type = "exp_zhao18",
end_cut = 60
)
#> Cutting measurements...
#> Estimating starting parameters for optimization...
#> Optimizing fitting parameters...
#> Calculating fits and slopes...
#> Done.
slopes_flag_df <- flux_quality(
slopes_df,
conc
)
#>
#> Total number of measurements: 6
#>
#> ok 6 100 %
#> discard 0 0 %
#> zero 0 0 %
#> force_discard 0 0 %
#> start_error 0 0 %
#> no_data 0 0 %
#> force_ok 0 0 %
#> force_zero 0 0 %
#> force_lm 0 0 %
#> no_slope 0 0 %
flux_plot(
slopes_flag_df,
conc,
datetime,
f_ylim_lower = 390,
f_ylim_upper = 650,
facet_wrap_args = list(
ncol = 3,
nrow = 2,
scales = "free"
)
)
#> Plotting in progress

Output of flux_plot, showing fluxes plotted individually with diagnostics and quality flags.
fluxes_df <- flux_calc(
slopes_flag_df,
f_slope_corr,
datetime,
temp_air,
conc_unit = "ppm",
flux_unit = "mmol/m2/h",
cols_keep = c("turfID", "type"),
cols_ave = c("temp_soil", "PAR"),
setup_volume = 24.575,
atm_pressure = 1,
plot_area = 0.0625
)
#> Cutting data according to 'keep_arg'...
#> Averaging air temperature for each flux...
#> Creating a df with the columns from 'cols_keep' argument...
#> Creating a df with the columns from 'cols_ave' argument...
#> Calculating fluxes...
#> R constant set to 0.082057 L * atm * K^-1 * mol^-1
#> Concentration was measured in ppm
#> Fluxes are in mmol/m2/h
fluxes_gpp <- flux_gpp(
fluxes_df,
type,
datetime,
id_cols = "turfID",
cols_keep = c("temp_soil_ave")
)
#> Warning in flux_gpp(fluxes_df, type, datetime, id_cols = "turfID", cols_keep = c("temp_soil_ave")):
#> NEE missing for measurement turfID: 156 AN2C 156
fluxes_gpp
#> # A tibble: 9 × 5
#> datetime type f_flux temp_soil_ave turfID
#> <dttm> <chr> <dbl> <dbl> <chr>
#> 1 2022-07-28 23:43:25 ER 51.9 10.9 156 AN2C 156
#> 2 2022-07-28 23:47:12 GPP 9.72 10.7 74 WN2C 155
#> 3 2022-07-28 23:47:12 NEE 32.0 10.7 74 WN2C 155
#> 4 2022-07-28 23:52:00 ER 22.3 10.7 74 WN2C 155
#> 5 2022-07-28 23:59:22 GPP -6.63 10.8 109 AN3C 109
#> 6 2022-07-28 23:59:22 NEE 44.3 10.8 109 AN3C 109
#> 7 2022-07-29 00:03:00 ER 50.9 10.5 109 AN3C 109
#> 8 2022-07-29 00:06:25 GPP NA 12.2 29 WN3C 106
#> 9 2022-07-29 00:06:25 NEE 32.7 12.2 29 WN3C 106
Supporting infrastructure
licoread
R package
The licoread
R package, developed in collaboration with LI-COR, provides an easy way to import raw files from LI-COR gas analyzers as R objects that can be used directly with the fluxible
R package.
Further developments
Segmentation tool
We are working on a tool to automatically select the window of the measurement on which to fit a model. This selection will be based on environmental variable, such as photosynthetically active radiation (PAR), or residuals.
Working in mol/volume
So far fluxible
works in fractional concentration (e. g. ppm) and transforms it in mol when calculating the fluxes, using the average temperature of the measurement. This has the advantage to work even if the setup does not provide temperature for each gas concentration data point. Recent setups provide temperature at the same frequency as gas concentration, and this allows to transform the concentration in mol/volume earlier in the process, accounting better for temperature changes during the measurement. This will be implemented in a future version of fluxible
.
Dissemination
If you are running a course and want to talk about fluxible
, feel free to use this two-slides presentation. Of course, you can always reach out if you wish to have more material.
Gaudard J, Chacon-Labella J, Dawson HR, Enquist B, Telford RJ, Töpper JP, Trepel J, Vandvik V, Baumane M, Birkeli K, Holle MJM, Hupp JR, Santos-Andrade PE, Satriawan TW, Halbritter AH. “fluxible
: an R package to process ecosystem gas fluxes from closed-loop chambers in an automated and reproducible way.” Authorea Preprints. doi:10.22541/au.175071021.14153294/v1, 2025.
Gaudard J, Trepel J, Dawson HR, Enquist B, Halbritter AH, Mustri M, Niittynen P, Santos-Andrade PE, Topper JP, Vandvik V, and Telford RJ. “fluxible
: an R package to calculate ecosystem gas fluxes from closed loop chamber systems in a reproducible and automated workflow” (slides), EGU General Assembly 2025, Vienna, Austria, 27 Apr–2 May 2025, EGU25-12409, doi:10.5194/egusphere-egu25-12409, 2025.
Gaudard J, Telford R, Vandvik V, and Halbritter AH: “fluxible
: an R package to calculate ecosystem gas fluxes in a reproducible and automated workflow” (poster), EGU General Assembly 2024, Vienna, Austria, 14–19 Apr 2024, EGU24-956, doi:10.5194/egusphere-egu24-956, 2024.
Acknowledgements
fluxible
builds on the earlier effort from the Plant Functional Traits Course Community co2fluxtent
(Brummer et al., 2023).